Locus Specific Probe for AFF1 Gene 3' Region    

The AFF1 3’ FISH Probe is designed to detect the human AFF1 gene 3’-region located on chromosome band 4q21.3. AFF1 is also known as AF4, PBM1 or MLLT2.

The AFF1 3' FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.

The AFF1 3' FISH probe hybridizes to chromosome 4 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 4q21.3 region on the chromosome 4. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 4q21.3. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 4.

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Analyte Specific Reagent. Analytical and performance characteristics are not established.