The AFF1 Break Apart FISH Probe Kit is designed to detect rearrangements in the human AFF1 gene located on chromosome band 4q21.3.* In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes, the probe set can also be used to identify other AFF1 aberrations such as deletions or amplifications. Rearrangements and abnormal expression of the AFF1 gene – also known as AF4, PBM1 or MLLT2 – have been observed in B-cell acute lymphocytic leukemias and numerous other hematological malignancies.

The probe is labeled with CytoOrange and CytoGreen. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with an appropriate filter; CytoGreen has an excitation peak at 495nm and emission peak at 518nm, giving it an green fluorescent signal with an appropriate filter.

* CytoTest shall not be responsible for patent infringement or any other violation that may occur with the use of this product.