| >> Weak or invisible signals |
| Possible Causes |
Suggested Solutions |
| Probe expired or quenched |
|
- Check the expiration date of the probes.
- Store the probes at -20 degree Celsius in the dark.
| |
| Air bubbles in the hybridization area |
|
- Clean the cover slips before use.
- Apply cover slips carefully to avoid making bubbles.
| |
| Probe solution dried out during hybridization |
|
- Make sure the cover slip is sealed by rubber cement completely.
- Add sufficient water in the moist chamber.
| |
| Inadequate denaturing of probes |
|
- Make sure the porbes are denatured at 75 degree Celsius.
| |
| Probes are too dilute |
|
- Use more concentrated probes.
| |
| Inadequate denaturing of slides |
|
- Increase the denaturing temperature.
- Increase the denaturing time.
- Change denaturing solution.
| |
| Incorrect filter used |
| (i.e. TexasRed filter should not be used for TAMRA)
- Select proper filters.
- Select proper color of the probes for the filters.
| |
| Exhausted filters |
|
- Change the filters according to the product instructions.
| |
| Exhausted mercury lamp |
|
- Change the mercury lamp after the lamp has been used for more than 200 hours.
| |
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