This CytoTest FISH probe set is designed to simultaneously determine the copy number of human chromosomes 13, 18 and 21, and to detect copy number aberrations in these chromosomes, in metaphase and interphase blood and tissue cells. The panel contains three different probes:

CCP18 is derived from chromosome 18 specific centromeric (18p11.1-q11.1) alpha satellite DNA (D18Z1).

CCP13 is a probe spanning the RB1 gene on 13q14 and adjacent sequences.

CCP21 is a probe covering the DSCR8 gene locus located on chromosome band 21q22.2.

The probe is labeled with CytoOrange, CytoGreen and CytoAqua. CytoOrange is a fluorophore with an excitation peak at 551 nm and emission peak at 575 nm, giving it an orange fluorescent signal with an appropriate filter; CytoGreen has an excitation peak at 495 nm and emission peak at 518 nm, giving it an green fluorescent signal with an appropriate filter; CytoAqua has an excitation peak at 422 nm and emission peak at 471 nm, giving it an aqua fluorescent signal with an appropriate filter.