This CytoTest FISH probe set is designed to simultaneously determine the copy number of human chromosomes 13 and 18, and to detect copy number aberrations in these chromosomes, in metaphase and interphase blood and tissue cells. The panel contains two different probes:

CCP18 is derived from chromosome 18 specific centromeric (18p11.1-q11.1) alpha satellite DNA (D18Z1).

CCP13 is a probe spanning the RB1 gene on 13q14 and adjacent sequences.

The probe is labeled with CytoOrange and CytoGreen. CytoOrange is a fluorophore with an excitation peak at 551 nm and emission peak at 575 nm, giving it an orange fluorescent signal with an appropriate filter; CytoGreen has an excitation peak at 495 nm and emission peak at 518 nm, giving it an green fluorescent signal with an appropriate filter.