The NTRK2 Break Apart FISH Probe Kit is designed to detect rearrangements in the human NTRK2 gene located on chromosome band 9q21.33. In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes, the probe set can also be used to identify other NTRK2 aberrations such as deletions or amplifications. Rearrangements and abnormal expression of the NTRK2 gene – also known as OBHD, TRKB, trk-B, EIEE58 or GP145-TrkB – has been observed in neuroblastoma, pancreatic ductal adenocarcinoma, Wilms’ tumors and colorectal cancer, and a number of developmental and metabolic disorders. 

The probe is labeled with CytoOrange and CytoGreen. CytoOrange is a fluorophore with an excitation peak at 551 nm and emission peak at 575 nm, giving it an orange fluorescent signal with an appropriate filter; CytoGreen has an excitation peak at 495 nm and emission peak at 518 nm, giving it an green fluorescent signal with an appropriate filter.