Product Description
CytoTest FISH probes are manufactured with genomic DNA obtained either from microdissected human chromosomes or cloned DNA fragments, depending on the probe type. The probes are labeled with the following fluorophores. The maximum peaks of excitation and emission wavelength for each dye and DAPI (Dihydrochloride 4',6'-Diamidino-2-phenylindole) counterstain are listed below. For optimal results, microscope filter sets must be selected which are compatible with the fluorescence of the probes.
Fluorophore
|
Excitation Peak (nm) |
Emission Peak (nm) |
Compatibility with Other Dyes |
CytoRedTM |
583 |
605 |
SpectrumRed
Propidium iodide (543-614) |
CytoOrangeTM |
551 |
575 |
SpectrumOrange |
CytoGoldTM |
523 |
549 |
SpectrumGold |
CytoGreenTM |
495 |
518 |
SpectrumGreen
FITC |
CytoAquaTM |
422 |
471 |
SpectrumAqua |
DAPI |
358 |
461 |
|
Warning & Precaution
Excessive exposure to light can cause the probe fluorophores to photobleach. Please take appropriate precautions when handling all reagents and slides containing probes to avoid direct and prolonged light exposure.
Storage
Store the FISH probes at -20 degree Celsius in the dark. Avoid repeated freeze/thaw cycles. Please check the expiration date on the product label before use.
Quality Control
All CytoTest FISH probes are tested by in situ hybridization to normal human lymphocytes metaphase spreads prior to shipping. |