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FISH Procedure for Cytological Specimens

Reagents Required but Not Provided

·         FISH Reagent Kit (Cat No: CT-ACC101-20):

o    20X SSC Salt: store at RT, avoid humidity

o    DAPI Counterstain: store at 4°C in the dark

o    NP-40: store at RT

·         Pepsin (Lyophilized): store at -20°C or below

·         Hydrochloric acid (1N): store at RT

·         Formaldehyde (37%): store at RT

·         10X Phosphate-buffered Saline (PBS) Solution: store at RT

·         Ethanol (100%): store at RT

 

Preparation of Working Solutions

1.     20X SSC Solution (pH 7.0)

Reagents

Amount added

Final Concentration

SSC Salt

66 g

20X

dH2O

250 ml

 

TOTAL

250 ml

 

 

2.     Pepsin Stock Solution

Reagents

Amount added

Final Concentration

Pepsin, lyophilized

100 mg

100 mg/ml

dH2O

1 ml

 

 
   

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