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   All chromosome slides that I have prepared have a halo around the chromosomes or cells. What could have caused such phenomenon?
   What kind(s) of chromosome FISH slide is most suitable for the FISH experiment?
   How important is pre-treating of paraffin-embedded slides?
   If the coverslip and the slide are not completely sealed by the rubber cement, will it affect the experiment result?
   After placing probe on the sample, how do I prevent bubbles from forming when placing the coverslip over the slide?
   During FISH hybridization, how should I place the slides in 37°C oven or incubator?
   How long should hybridization be performed?
   How long can the denature solution be used?
   What can happen if the sample slides are under- or over-denatured?
   What are the most critical factors for denaturing?
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