USA    |    Global :  English    简中    繁中    |    MAP
  Login    |     Shopping Cart
 
Technologies
  FISH
  CISH/SISH
  CGH
  SKY
  Links
 
  CGH

Comparative Genomic Hybridization (CGH) 

Comparative genomic hybridization (CGH) is a molecular cytogenetic technique designed to measure DNA copy number changes such as the gains or losses of specific DNA regions often observed in tumor cells. In CGH, two probe pools, one derived from tumor DNA, the other from normal DNA fragments, are labeled with two different fluorochromes, mixed and hybridized to normal metaphase chromosomes. Localized gains or losses of DNA result in increased or decreased fluorescence intensity along a chromosome.

CGH is a sensitive method and can detect quantitative chromosomal changes with high resolution, especially when applied in a microarray format. It is then sometimes referred to as Chromosomal Microarray Analysis (CMA). CGH detects rearrangements resulting in a net loss or gain of specific regions. However, it cannot reveal polyploidy changes (increase in number of the entire chromosome set) or chromosomal aberrations in which the total DNA amount remains the same, such as balanced translocations or inversions. Such chromosomal rearrangements can be visualized by Spectral Karyotyping.

 

CytoTest Inc.  |  9430 Key West Avenue, Suite 210, Rockville, MD 20850, USA
Tel:1-855-CYTOTEST  |  +1-202-688-1188  |  sales@cytotest.com

© 2013 CytoTest Inc. All Rights Reserved.
Website Design | Youhome